Environmental DNA (eDNA) is becoming a mainstay of non-invasive, reliable, and cost-effective surveys of at-risk and invasive species, pathogen detection, and biodiversity assessment. These approaches rely on the fact that organisms leave their DNA in the environment, making it possible to detect their presence without capture or even direct observation. Reliable eDNA data depend on field and lab rigorous technique because significant biases can occur through the process from the sampling to the data processing, and they greatly influence species…